Isolation and characterization of canine cardiac sarcoplasmic reticulum with improved Ca2+ transport properties.

@article{Chamberlain1983IsolationAC,
  title={Isolation and characterization of canine cardiac sarcoplasmic reticulum with improved Ca2+ transport properties.},
  author={B K Chamberlain and Dmitri O. Levitsky and Sidney Fleischer},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 10},
  pages={6602-9}
}
A procedure has been developed for isolating canine cardiac sarcoplasmic reticulum with considerably improved Ca2+ transport properties and stability. Contamination by mitochondria and sarcolemma is low, and the preparation is at least 85% pure sarcoplasmic reticulum. The preparation exhibits efficient, high activity ATP-dependent, oxalate-facilitated Ca2… CONTINUE READING