Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments.

@article{Baehr1979IsolationAC,
  title={Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments.},
  author={Wolfgang Baehr and Michael J. Devlin and Meredithe L. Applebury},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 22},
  pages={11669-77}
}
A simple purification method has been developed for isolation of bovine cGMP phosphodiesterase from photoreceptor rod outer segments. The enzyme is peripherally membrane bound in its native state and is present in relatively high concentrations. In the bovine photoreceptors its molar ratio to rhodopsin can be estimated to be as great as 1:40 and not lower… CONTINUE READING