Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia.

@article{Guyon2000IsolationAC,
  title={Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia.},
  author={Virginie N. Guyon and James D. Astwood and Ethan C Garner and A. Keith Dunker and Loverine P. Taylor},
  journal={Plant physiology},
  year={2000},
  volume={123 2},
  pages={699-710}
}
Petunia (Petunia hybrida) pollen requires flavonols (Fl) to germinate. Adding kaempferol to Fl-deficient pollen causes rapid and synchronous germination and tube outgrowth. We exploited this system to identify genes responsive to Fls and to examine the changes in gene expression that occur during the first 0.5 h of pollen germination. We used a subtracted library and differential screening to identify 22 petunia germinating pollen clones. All but two were expressed exclusively in pollen and… CONTINUE READING

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