Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase.

@article{Olsen1991IsolationAC,
  title={Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase.},
  author={Sue Olsen and Michael Uhler},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 17},
  pages={11158-62}
}
Synthetic oligonucleotides were designed to amplify DNA sequences related to the heat-stable protein kinase inhibitor (PKI) isolated from rabbit skeletal muscle. Using these oligonucleotides, a 167-base pair fragment was isolated and shown to code for a portion of the mouse protein kinase inhibitor gene. This amplified DNA sequence was used to isolate three cDNA clones from a mouse brain cDNA library. A composite sequence was derived from these clones and contained a 228-nucleotide open reading… CONTINUE READING