Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.

  title={Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.},
  author={Elena A Jones and S. E. Kinsey and Anne English and Richard G. Jones and Liz Straszynski and David M. Meredith and Alex F. Markham and Andrew Jack and Paul Emery and Dennis McGonagle},
  journal={Arthritis and rheumatism},
  volume={46 12},
OBJECTIVE There is an increased interest in rheumatology in mesenchymal progenitor/stem cells (MPCs) and their roles in rheumatic diseases, but little is known about the phenotype of these cells in vivo. The aim of this study was to isolate and characterize human bone marrow (BM) MPCs. METHODS Fluorescence microscopy was used to identify putative MPCs among adherent BM cells. To purify them, a positive selection with antifibroblast microbeads was used, combined with fluorescence-activated… CONTINUE READING
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