Isolation and characterization of an arterivirus defective interfering RNA genome.

@article{Molenkamp2000IsolationAC,
  title={Isolation and characterization of an arterivirus defective interfering RNA genome.},
  author={Richard Molenkamp and B C Rozier and Sophie Greve and Willy J. M. Spaan and Eric J. Snijder},
  journal={Journal of virology},
  year={2000},
  volume={74 7},
  pages={3156-65}
}
Equine arteritis virus (EAV), the type member of the family Arteriviridae, is a single-stranded RNA virus with a positive-stranded genome of approximately 13 kb. EAV uses a discontinuous transcription mechanism to produce a nested set of six subgenomic mRNAs from which its structural genes are expressed. We have generated the first documented arterivirus defective interfering (DI) RNAs by serial undiluted passaging of a wild-type EAV stock in BHK-21 cells. A cDNA copy of the smallest DI RNA (5… CONTINUE READING

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