Isolation and characterization of a vinculin cDNA from chick-embryo fibroblasts.

@article{Price1987IsolationAC,
  title={Isolation and characterization of a vinculin cDNA from chick-embryo fibroblasts.},
  author={Glenda Price and Peter Jones Jones and Matthew D. Davison and Bhavesh K. Patel and Ian C. Eperon and David R. Critchley},
  journal={The Biochemical journal},
  year={1987},
  volume={245 2},
  pages={
          595-603
        }
}
A chick-embryo fibroblast lambda gt11 cDNA library was screened with affinity-purified antibodies to chick gizzard vinculin. One recombinant was purified to homogeneity and the fusion protein expressed in Escherichia coli strain C600. The fusion protein was unstable, but polypeptides that reacted with vinculin antibodies, but not non-immune immunoglobulin, were detected by Western blotting. The recombinant contained a single EcoRI fragment of 2891 bp with a single open reading frame. The… CONTINUE READING
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