Isolation and characterization of a soluble antigen complex of Plasmodium falciparum with pyrogenic properties.

Abstract

A soluble antigen complex, previously designated antigen no. 7 (Ag7) on the basis of the pattern obtained by crossed immunoelectrophoresis of culture supernatants of P. falciparum, was isolated by affinity chromatography. It was shown to be synthesized at the schizont stage of the parasite growth cycle and to be located on the surface of the schizonts. Antibodies to Ag7 did not inhibit the growth of the parasite in vitro. Ag7 is recognized by immune human sera from many parts of the world and it stimulated the production of specific antibody in mice when incorporated into immune-stimulating complex (ISCOM) structures. It also specifically stimulated in vitro proliferation of lymphocytes from clinically immune adults. That it induced the secretion of interleukin 1 by human monocytes and was pyrogenic in rabbits was of particular interest. Thus Ag7 has endotoxin-like properties which make it a possible candidate for an antitoxic malaria vaccine.

Cite this paper

@article{Jakobsen1991IsolationAC, title={Isolation and characterization of a soluble antigen complex of Plasmodium falciparum with pyrogenic properties.}, author={Palle H. Jakobsen and Lars Hviid and Thor G. Theander and Eleanor M. Riley and Philippe Grellier and Lene S\ondberg Bruun and Kristian Dalsgaard and Joseph Schr{\'e}vel and S\oeren Jepsen}, journal={APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}, year={1991}, volume={99 1}, pages={21-9} }