Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells.

Abstract

We have extensively purified from Krebs II ascites cells, although not until homogeneity, a ribonuclease which preferentially cleaves natural or synthetic double-stranded RNA substrates (RNase D); this specificity is also supported by its sensitivity to inhibition by 10(-5) M ethidium bromide. It does not degrade RNA-DNA hybrids and is, therefore, clearly… (More)

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Cite this paper

@article{Rech1980IsolationAC, title={Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells.}, author={Jocelyne Rech and G. Cathala and P. Jeanteur}, journal={The Journal of biological chemistry}, year={1980}, volume={255 14}, pages={6700-6} }