Isolation and characterization of a reservosome fraction from Trypanosoma cruzi.

@article{CunhaESilva2002IsolationAC,
  title={Isolation and characterization of a reservosome fraction from Trypanosoma cruzi.},
  author={Narcisa L Cunha-E-Silva and Georgia C Atella and Isabel A Porto-Carreiro and Jose Andr{\'e}s Morgado-D{\'i}az and Miria G Pereira and Wanderley de Souza},
  journal={FEMS microbiology letters},
  year={2002},
  volume={214 1},
  pages={
          7-12
        }
}
Reservosomes are acidic compartments present at the posterior region of epimastigote forms of Trypanosoma cruzi that store proteins and lipids. During metacyclogenesis, they consume their contents and disappear. Reservosomes are rich in cruzipain, the main proteolytic enzyme of this parasite. By centrifugation in a sucrose gradient, we have obtained a highly purified subcellular fraction containing reservosomes from 5-day-old Y strain epimastigotes. Transmission electron microscopy showed that… CONTINUE READING
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