Isolation and characterization of a rabbit epididymal secretory glycoprotein that associates to the spermatozoon surface.

Abstract

Using a combination of gel filtration and hydroxyapatite chromatography, a major secretory glycoprotein (EP140) was purified from rabbit epididymal fluid. The protein had an apparent Mr of approximately 140 kDa under native conditions but dissociated into 2 equimolar amounts of glycosylated subunits, alpha and beta of Mr 35 and 33 kDa, upon sodium… (More)

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