Isolation and characterization of a platelet-derived macrophage-binding proteoglycan.

  title={Isolation and characterization of a platelet-derived macrophage-binding proteoglycan.},
  author={Jaime Mas-Oliva and Kay S. Arnold and William D. Wagner and D. Ruiz-Tagle Phillips and Robert E. Pitas and Thomas L. Innerarity},
  journal={The Journal of biological chemistry},
  volume={269 13},
A macromolecule in human platelet secretory products was demonstrated previously to inhibit the binding and uptake of acetoacetylated (AcAc) low density lipoproteins (LDL) by scavenger receptors on mouse peritoneal macrophages. In the current study, this macromolecule was purified to apparent homogeneity by DEAE-Sephacel chromatography, Sephacryl S-300 chromatography, and sucrose gradient centrifugation. SDS-polyacrylamide gel electrophoresis revealed a single band with an apparent molecular… CONTINUE READING

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