Isolation and characterization of a feeder-dependent, porcine trophectoderm cell line obtained from a 9-day blastocyst.

@article{Flechon1995IsolationAC,
  title={Isolation and characterization of a feeder-dependent, porcine trophectoderm cell line obtained from a 9-day blastocyst.},
  author={Jacques E. Flechon and Sophie A. Laurie and E. Notarianni},
  journal={Placenta},
  year={1995},
  volume={16 7},
  pages={643-58}
}
We have established in culture a feeder-dependent cell line, termed TE1, from a 9 day, pre-implantation, porcine embryo. TE1 cells were observed by light and electron microscopy, and characterized by immunocytochemistry: the morphology, cytology and ultrastructure of this cell line are described. The cells display epithelial characteristics, as revealed using immunofluorescence microscopy with antibody against cytokeratins of simple epithelia, but not with antibody against vimentin. The cells… CONTINUE READING

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