Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota).

@article{Smallwood1999IsolationAC,
  title={Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota).},
  author={Maggie Smallwood and Dawn Worrall and Louise Byass and Luisa Elias and Dave Ashford and Charlotte J Doucet and Carl Holt and Julia Telford and Peter J Lillford and Dianna J. Bowles},
  journal={The Biochemical journal},
  year={1999},
  volume={340 ( Pt 2)},
  pages={385-91}
}
A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants. This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N-glycosylated. The corresponding gene is unique in the… CONTINUE READING

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