Isolation and characterization of a novel oxidant- and surfactant-stable extracellular alkaline protease from Exiguobacterium profundum BK-P23.

@article{Anbu2013IsolationAC,
  title={Isolation and characterization of a novel oxidant- and surfactant-stable extracellular alkaline protease from Exiguobacterium profundum BK-P23.},
  author={Periasamy Anbu and Byung Ki Hur and Choul Gyun Lee},
  journal={Biotechnology and applied biochemistry},
  year={2013},
  volume={60 2},
  pages={155-61}
}
The novel protease from Exiguobacterium profundum BK-P23 was partially purified by ammonium sulfate precipitation and further purified Mono Q 5/50 and Superdex 200 10/300 column chromatography. The enzyme was purified 10.23-fold with a yield of 14%. The molecular weight was estimated to be 52 kDa by SDS-PAGE. The enzyme was most active at a pH of 8.0 and… CONTINUE READING