Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

@article{Yokota1984IsolationAC,
  title={Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.},
  author={T. Yokota and Frank Lee and Donna M. Rennick and C. V. Hall and Naoko Arai and Tim R. Mosmann and Gary J. Nabel and Harvey Cantor and Ken-ichi Arai},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 4},
  pages={
          1070-4
        }
}
  • T. Yokota, F. Lee, K. Arai
  • Published 1 February 1984
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF… 
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References

Molecular Cloning, a Laboratory Manual (Cold Spring Harbor Laboratory
  • 1982