• DOI:10.1073/PNAS.81.4.1070
• Corpus ID: 44648117

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

@article{Yokota1984IsolationAC,
  title={Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.},
  author={T. Yokota and Frank Lee and Donna M. Rennick and C. V. Hall and Naoko Arai and Tim R. Mosmann and Gary J. Nabel and Harvey Cantor and Ken-ichi Arai},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 4},
  pages={
          1070-4
        }
}

• T. Yokota, F. Lee, +6 authors K. Arai
• Published 1 February 1984
• Biology, Chemistry
• Proceedings of the National Academy of Sciences of the United States of America

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.