Isolation and characterization of a genomic sequence encoding the maize Cat3 catalase gene.

@article{Abler1993IsolationAC,
  title={Isolation and characterization of a genomic sequence encoding the maize Cat3 catalase gene.},
  author={Michael L. Abler and John G. Scandalios},
  journal={Plant molecular biology},
  year={1993},
  volume={22 6},
  pages={1031-8}
}
A maize genomic DNA library (Zea mays L. W64A) was constructed using the lambda GEM-11 vector. A gene-specific Cat3 cDNA probe was used to screen the library by plaque hybridization. Two independent clones, lambda 24C and lambda 27C, which hybridized to the Cat3 cDNA probe, were isolated. Restriction maps were created for the two clones (lambda 24C and lambda 27C) encoding the maize Cat3 catalase gene. A Sal I restriction fragment from each clone was subcloned into pBluescript KS+. Subsequent… CONTINUE READING

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