Isolation and characterization of a genomic clone encoding the β-subunit of β-conglycinin


β-Conglycinin, and abundant storage protein in soybean (Glycine max (L.) Merr.) seeds, is a trimeric protein consisting of various isomers containing the three subunits, α′, α and β. Accumulation of the β-subunit is unique because it appears to be regulated by a variety of developmental and environmental signals. In this paper we describe the isolation and characterization of a genomic clone encoding the β-subunit of β-conglycinin. The genomic clone was characterized by restriction-enzyme mapping and partial DNA sequence analysis, by immunoprecipitation of a hybrid-selected invitro translation product, and by RNA blot hybridization reactions. An mRNA of approx. 1700 nucleotides hybridized to an internal 2-kilobase (kb) region of this 4.4-kb cloned DNA restriction fragment and was translated to yield a polypeptide with an approximate molecular weight of 48 kilodalton. This polypeptide is immunoprecipitable by antibody against β-conglycinin and is of appropriate size to represent the precursor polypeptide of the β-subunit. When this sequence was used as a probe in RNA blot hybridization experiments, the β-gene transcript was first detected by stage K and accumulated through stage O during soybean seed development, coincident with appearance of the β-subunit. Partial DNA sequence analysis of the 5′ end of the gene confirmed that the isolated gene encoded a β-subunit, based upon the previously reported amino terminal sequence for this protein. Genomic DNA blot hybridization analyses indicate that multiple DNA restriction fragments are highly homologous to this cloned β-gene sequence.

DOI: 10.1007/BF00398664

6 Figures and Tables


Citations per Year

153 Citations

Semantic Scholar estimates that this publication has 153 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Tierney1987IsolationAC, title={Isolation and characterization of a genomic clone encoding the β-subunit of β-conglycinin}, author={Mary Lou Tierney and Elizabeth Ann Bray and Randy D Allen and Y T Ma and Roger F. Drong and Jerry L. Slightom and Roger N. Beachy}, journal={Planta}, year={1987}, volume={172}, pages={356-363} }