Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase.

@article{Choi1997IsolationAC,
  title={Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase.},
  author={Hong Keun Choi and Kwang Ho Choi and Karl J. Kramer and Subbaratnam Muthukrishnan},
  journal={Insect biochemistry and molecular biology},
  year={1997},
  volume={27 1},
  pages={37-47}
}
Genomic clones for a chitinolytic enzyme were isolated from a library of Sau 3A digested DNA from the tobacco hornworm, Manduca sexta, using a previously isolated chitinase cDNA clone as a probe [Kramer et al., Insect Biochem. Molec. Biol. 23, 691-701 (1993)]. Restriction enzyme mapping and Southern blot analysis of four genomic clones suggested that these are overlapping clones. Sequence analysis of the genomic clones and Southern blot analysis of total genomic DNA also suggest that the M… CONTINUE READING