Isolation and characterization of a carboxysome shell gene from Thiobacillus neapolitanus.

@article{English1994IsolationAC,
  title={Isolation and characterization of a carboxysome shell gene from Thiobacillus neapolitanus.},
  author={Rowena English and S. C. Lorbach and Xiaoting Qin and Jessup M. Shively},
  journal={Molecular microbiology},
  year={1994},
  volume={12 4},
  pages={647-54}
}
The gene coding for the major carboxysome shell peptide (csoS1) from Thiobacillus neapolitanus has been isolated and sequenced. Oligonucleotide primers for polymerase chain reaction (PCR) amplification of the 5' end of the gene were made possible by amino acid sequencing of the N-terminal residues of the shell peptide. A 41 bp PCR product was used as a probe to isolate the gene. The deduced amino acid composition of the 216 bp gene shows a high degree of hydrophobicity. The gene is located… CONTINUE READING
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