Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase.

@article{Kimura1990IsolationAC,
  title={Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase.},
  author={Narimichi Kimura and Noriaki Shimada and Kinya Nomura and Katsuto Watanabe},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 26},
  pages={15744-9}
}
A cDNA clone for cytosolic nucleoside diphosphate (NDP) kinase was isolated from a cDNA library of rat skeletal muscle using synthetic oligonucleotides as probes. The clone constitutes a 621-base pair cDNA sequence including the 456-base pair coding region and 137-base pair 3'-untranslated one with polyadenylation site. The complete primary structure of NDP kinase was deduced from the coding sequence. An NH2-terminal amino acid sequence analysis suggested that the translated enzyme protein… CONTINUE READING

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