Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L.

@article{Davies1993IsolationAC,
  title={Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L.},
  author={Kevin M Davies and Geoffrey A. M. King},
  journal={Plant physiology},
  year={1993},
  volume={102 4},
  pages={
          1337-40
        }
}
A full-length cDNA clone (pTIP27) encoding asparagine synthetase (AS; EC 6.3.5.4) was isolated from a cDNA library prepared from the tip section (apex to 30 mm) of Asparagus officinalis L. spears. The cDNA clone encodes an mRNA of 1978 bp, giving a derived protein of 66.5 kD molecular mass. The derived amino acid sequence is 81% homologous to AS from Pisum sativum. Only low levels of transcript for AS could be detected in growing spears, roots, or ferns. However, AS mRNA levels began to… CONTINUE READING
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