Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst

@article{Talbot2004IsolationAC,
  title={Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst},
  author={Neil C Talbot and Thomas J. Caperna and ANNE M. Powell and Wesley M. Garrett and Alan D. Ealy},
  journal={Molecular Reproduction and Development},
  year={2004},
  volume={69}
}
A bovine trophectoderm cell line was established from a parthenogenetic in vitro‐produced blastocyst. To initiate the cell line, 8‐day parthenogenetic blastocysts were attached to a feeder layer of STO fibroblasts and primary outgrowths occurred that consisted of trophectoderm, endoderm, and very occasionally epiblast tissue. Any endoderm and epiblast outgrowths were removed from the primary cultures within the first 10 days of culture by dissection. One of the primary trophectoderm cell… Expand
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