Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst

  title={Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst},
  author={Neil C Talbot and Thomas J. Caperna and ANNE M. Powell and Wesley M. Garrett and Alan D. Ealy},
  journal={Molecular Reproduction and Development},
A bovine trophectoderm cell line was established from a parthenogenetic in vitro‐produced blastocyst. To initiate the cell line, 8‐day parthenogenetic blastocysts were attached to a feeder layer of STO fibroblasts and primary outgrowths occurred that consisted of trophectoderm, endoderm, and very occasionally epiblast tissue. Any endoderm and epiblast outgrowths were removed from the primary cultures within the first 10 days of culture by dissection. One of the primary trophectoderm cell… Expand
An Efficient System to Establish Biopsy-Derived Trophoblastic Cell Lines from Bovine Embryos1
A system to expand in vitro trophoblastic cells from an embryo biopsy is developed that solves the limitations of using amplified DNA from a small number of cells for bovine embryo genotyping and epigenotypes and facilitates the establishment of trophobastic cell lines that can be useful as peri-implantation in vitro models. Expand
Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors
Bovine trophectoderm can be induced via reprogramming factor expression from bovine liver‐derived fibroblasts, although other fibroblast populations—e.g., derived from fetal thigh tissue—may produce similar results, albeit at lower frequencies. Expand
Trophoblast stem cell marker gene expression in inner cell mass-derived cells from parthenogenetic equine embryos.
The results suggest that cells obtained after passages of primary ICM outgrowths are positive for trophoblast stem cell markers while expressing POU5F1 and displaying AP activity, therefore, these cells most likely represent trophOBlast cells rather than true ESCs. Expand
Establishment of a bovine blastocyst-derived cell line collection for the comparative analysis of embryos created in vivo and by in vitro fertilization, somatic cell nuclear transfer, or parthenogenetic activation
A collection of bovine blastocyst-derived cell lines was created to define better the nature of the nuclear reprogramming that occurs after NT, and the use of the trophectoderm cell lines as a comparative in vitro model ofbovine tropheCToderm and placental function is discussed in relation to NT reprograming. Expand
Comparison of the interferon‐tau expression from primary trophectoderm outgrowths derived from IVP, NT, and parthenogenote bovine blastocysts
Analysis of trophectoderm colony size after 23 days in culture showed a similar relationship with P‐derived colonies being significantly smaller in comparison to IVP and NT colonies, and a differential expression of IFN‐τ was observed again, but this time as measured over time in culture. Expand
Conversion of equine umbilical cord matrix mesenchymal stem cells to the trophectoderm lineage using the Yamanaka reprogramming factors
This report marks the generation of the first equine Trophoblast cell line capable of recapitulating early equine trophobasts development and could prove valuable in gaining greater understanding of equine trophectoderm development. Expand
Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation
An in-depth proteomic analysis of the secretome of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial–mesenchymal transition stages in these cells, found that the pattern of expression of implantation proteins in BBT-EVs changes depending on their epithelial-mesenchmal phenotype. Expand
Embryonic development and implantation related gene expression of oocyte reconstructed with bovine trophoblast cells.
Using BTs expressing IFNτ as donor cells for bovine NT could be a useful tool for understanding theIFNτ genetics and epigenetics. Expand
Parthenogenesis in non-rodent species: developmental competence and differentiation plasticity.
In an attempt to better elucidate some of these aspects, parthenogenetic cell lines, recently derived in the laboratory, have been characterized for their pluripotency, demonstrating the ability of these cells to differentiate into cell types derived from the three germ layers. Expand
Effect of origin and culture conditions on the heterogeneity of pluripotent cell populations
Las celulas pluripotentes son una poderosa herramienta para la medicina regenerativa y la manipulacion genetica. Sin embargo, aun se desconocen aspectos clave acerca de las condiciones optimas paraExpand


Bovine Blastocyst-Derived Trophectoderm and Endoderm Cell Cultures: Interferon Tau and Transferrin Expression as Respective In Vitro Markers
This simple coculture method for the in vitro propagation of bovine trophectoderm and endoderm provides a system for assessing their biology in vitro. Expand
Isolation and characterization of a bovine blastocyst-derived trophoblastic cell line, BT-1: development of a culture system in the absence of feeder cell.
The BT-1 trophoblastic cell line could serve as a powerful model system for the study of trophoblast cell lineage and proliferation and was found that the cell growth was accelerated in fibroblast-conditioned medium. Expand
Visceral-endoderm-like cell lines induce differentiation of murine P19 embryonal carcinoma cells.
Results demonstrated that aggregation was necessary, but not sufficient, to make P19 EC cells differentiate, and medium conditioned by cells of the END-2 line was particularly potent in inducing endodermal and mesodermal differentiation of single P19 aggregates, confirming the involvement of a diffusible factor secreted specifically by visceral-endoderm-like cells in this process. Expand
Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen
It is shown that a bovine trophoblastic cell line established from in vitro fertilized blastocysts differentiated into binucleate cells on collagen gel, which have features characteristic of those in vivo, including an increased nuclear DNA content and the expression of placental lactogen. Expand
A teratocarcinoma-derived endoderm stem cell line (1H5) that can differentiate into extra-embryonic endoderm cell types.
The 1H5 cell line could provide a useful system for studying the characteristics and mechanisms underlying visceral-endoderm differentiation in vitro, since it has the distinct advantage that homogeneous cultures are produced, in contrast to other teratocarcinoma cell lines such as F9 which differentiate into a mixture of cell types. Expand
In vitro pluripotency of epiblasts derived from bovine blastocysts
In vivo‐derived blastocysts, especially from early‐hatching blastocyst, were a superior source of pluripotent epiblasts, indicating that standard conditions for mouse embryonic stem cell culture do not maintain bovine epiblast cells in an undifferentiated state. Expand
Serological characterization of a pluripotent mouse embryonal stem cell line, two transformed derivatives, and an endoderm-like cell line.
The results obtained indicate that BLC 1, T1 and T2/K26 are undifferentiated embryonal stem cells, and BLC 3 is an endoderm-like cell line. Expand
Ultrastructural and karyotypic examination of in vitro produced bovine embryos developed in the sheep uterus.
In vivo incubation in the sheep uterus allowed nearly normal development to the elongated blastocyst stage and may be useful for assessment of NT bovine blastocysts developmental competence. Expand
Establishment of pluripotential cell lines from haploid mouse embryos.
Eggs from 129 SvE and (C57BL x CBA)F1 hybrid female mice activated parthenogenetically following their exposure to a 7% solution of ethanol in PBS provided a source of homozygous diploid cell lines of parthenogenic origin. Expand
Cultured stem‐cells from human testicular teratomas: The nature of human embryonal carcinoma, and its comparison with two types of yolk‐sac carcinoma
Human testicular teratomas consist of epithelial cells which may be nullipotent, pluripotent or committed to extraembryonic endodermal differentiation. Expand