Isolation and characterization of Sendai virus DI-RNAs

@article{Kolakofsky1976IsolationAC,
  title={Isolation and characterization of Sendai virus DI-RNAs},
  author={Daniel Kolakofsky},
  journal={Cell},
  year={1976},
  volume={8},
  pages={547-555}
}
When passaged at high multiplicity, four strains of Sendai virus all showed evidence that they contained defective interfering (DI) particles. RNA isolated from nucleocapsids of cells infected with the high multiplicity passage stocks was found to consist of only minor amounts of nondefective genome length RNA and major amounts of smaller RNAs, the DI-RNAs. These DI-RNAs were found to have unusual and variable sedimentation properties in sucrose gradients, but were found to represent unique… 
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Abstract We show that mixed infection with standard virus and defective interfering (DI) particles of Sendai virus regularly leads to the survival of infected cells and to the establishment of
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Intracellular stability of nonreplicating paramyxovirus nucleocapsids.
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It is concluded that the intracellular half-life of measles virus DI nucleocapsids makes possible DI replication in the human body after vaccination with a DI-contaminated attenuated live virus, even when this vaccination represents a low multiplicity of infection.
Molecular cloning of natural paramyxovirus copy-back defective interfering RNAs and their expression from DNA.
TLDR
Using the unique sequence organization of copy-back defective interfering (DI) RNAs of paramyxoviruses, Sendai virus, and measles virus copy- back DI RNAs were PCR amplified and cloned, opening the possibility of examining the cis-acting sequences involved in viral multiplication directly, without using indirect markers such as CAT activity.
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TLDR
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