Isolation and characterization of PSII core complexes from a brown alga, Laminaria saccharina.

@article{Douady1993IsolationAC,
  title={Isolation and characterization of PSII core complexes from a brown alga, Laminaria saccharina.},
  author={Dominique Douady and Bernard Rousseau and Claire Berkaloff},
  journal={FEBS letters},
  year={1993},
  volume={324 1},
  pages={22-6}
}
PSII-enriched particles, active for DCIP-reduction, were prepared from Laminaria saccharina chloroplasts, and PSII core complexes were further purified by ion-exchange chromatography. They contained several polypeptides, four of them cross-reacting with antibodies raised against CP47, CP43, D1 and D2 of green plants. A second chromatography was required to separate: (i) a core antenna, composed of 51 kDa polypeptide subunits, binding 11 beta-carotene, 4 chlorophyll (Chl) c and 7 fucoxanthin for… CONTINUE READING