Isolation and characterization of Neurospora crassa nucleoside diphosphate kinase NDK-1.

@article{Ogura1999IsolationAC,
  title={Isolation and characterization of Neurospora crassa nucleoside diphosphate kinase NDK-1.},
  author={Yusuke Ogura and Yasuhiro Yoshida and Kazuyoshi Ichimura and Chika Aoyagi and Naoto Yabe and Kohji Hasunuma},
  journal={European journal of biochemistry},
  year={1999},
  volume={266 3},
  pages={709-14}
}
We have previously reported that phosphorylation of a 15-kDa protein increased after blue-light irradiation in Neurospora crassa. In this study, the 15-kDa protein was purified using four columns; DEAE-cellulose, Blue-Sepharose, SP-Sepharose and Mono Q. The 15-kDa protein was shown to be homologous with nucleoside diphosphate kinase by amino acid sequencing and was also shown to possess nucleoside diphosphate kinase activity. A gene encoding N. crassa nucleoside diphosphate kinase, ndk-1, was… CONTINUE READING
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