Isolation and characterization of Kupffer and endothelial cells from the rat liver.

  title={Isolation and characterization of Kupffer and endothelial cells from the rat liver.},
  author={Dick L. Knook and N Blansjaar and E. Ch. Sleyster},
  journal={Experimental cell research},
  volume={109 2},

Primary cultures of endothelial cells of the rat liver

From the results obtained in this study, maintenance culture of rat liver endothelial cells seems to be a promising system for studying the regulation of pore size of the fenestrated sieve plates by alcohol and certain hormones, for study the interaction of endothelium cells with other liver cells and tumor cells, and for studied the mechanisms of adsorptive endocytosis.

Preparation of Pure Hepatocytes and Reticuloendothelial Cells in High Yield From a Single Rat Liver by Means of Percoll Centrifugation and Selective Adherence

Ulastructural analysis shows that Percoll does not associate with the cells during the separation procedure, and the technique is based on collagenase perfusion of the liver, isopycnic sedimentation in Percoll, and selective adherence of the cells.

Isolation of oval cells by centrifugal elutriation and comparison with other cell types purified from normal and preneoplastic livers.

Nucleic acid hybridization using specific probes revealed that oval cells and biliary epithelial cells isolated from livers of rats fed a choline-deficient diet contain albumin and alpha-fetoprotein messenger RNAs, while hepatocytes from normal and preneoplastic liver contain only albumin messenger RNA.

Sinusoidal Endothelial Cells from Normal Guinea Pig Liver: Isolation, Culture and Characterization

These methods for isolating and culturing sinusoidal endothelial cells should permit further functional assessment of endothelium cells and their interrelationship with other sinusoid lining cells.

Hepatocyte suspensions and cultures as tools in experimental carcinogenesis.

  • P. Seglen
  • Biology, Medicine
    Journal of toxicology and environmental health
  • 1979
Density gradient separation or selection in culture on the basis of the unique properties of preneoplastic hepatocytes (e.g., drug resistance) may prove useful and the use of hepatocyte cultures and liver-derived epithelial cell lines as test systems and models for chemical carcinogenesis in vitro is briefly reviewed.



Mass isolation and culture of rat kupffer cells

Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer

Identity and activities of lysosomal enzymes in parenchymal and non-parenchymal cells from rat liver.

It can be concluded that the non-parenchymal cells possess a set of lysosomal enzymes which makes them extremely suitable for a phagocytic and antimicrobial function in the liver.


Assay of the MHO activity of the whole spleen and of the total EP cell population of the liver suggested that these two tissues may be of comparable importance in their ability to degrade RBC-hemoglobin.


Kupffer cells obtained under such conditions are abnormally active, and some can be washed out of the liver of sick animals in the absence of any preliminary phagocytosis of particulate matter.