Isolation and characterization of Kluyveromyces marxianus mutants deficient in malate transport.

@article{Queirs1998IsolationAC,
  title={Isolation and characterization of Kluyveromyces marxianus mutants deficient in malate transport.},
  author={Od{\'i}lia Queir{\'o}s and Margarida Casal and Steven Althoff and Pedro Moradas-Ferreira and Cec{\'i}lia Sued Le{\~a}o},
  journal={Yeast},
  year={1998},
  volume={14 5},
  pages={401-7}
}
In malic acid-grown cells of the strains ATCC 10022 and KMS3 of Kluyveromyces marxianus the transport of malic acid occurred by a malate-proton symport, which accepted L-malic, D-malic, succinic and fumaric acids, but not tartaric, malonic or maleic acids. The system was inducible and subjected to glucose repression. Mutants of the strain KMS3, unable to grow in a medium with malic acid, were isolated and checked for their capacity to utilize several carbon sources and to transport dicarboxylic… CONTINUE READING