Isolation and characterization of IS1 circles.

  title={Isolation and characterization of IS1 circles.},
  author={Yasuji Sekine and Naoki Eisaki and Kazuyasu Kobayashi and Eiichi Ohtsubo},
  volume={191 2},
Transposase encoded by insertion sequence IS1 is produced from two out-of-phase reading frames by translational frameshifting that occurs in a run of adenines. An IS1 mutant with a single adenine insertion in the run of adenines efficiently produces transposase, resulting in generation of miniplasmids by deletion for a region adjacent to IS1 from a plasmid carrying the IS1 mutant. Here, we found that besides miniplasmids, cells harboring the plasmid contained minicircles without the region… CONTINUE READING