Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.

@article{Vakharia2001IsolationAC,
  title={Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.},
  author={Hema Vakharia and Greg J German and Rajeev Misra},
  journal={Journal of bacteriology},
  year={2001},
  volume={183 23},
  pages={
          6908-16
        }
}
This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identical to that in the parental strain, thus reflecting a normal intracellular toxin activation event. Treatment of the secreted toxin with guanidine hydrochloride significantly restored… CONTINUE READING
Highly Cited
This paper has 22 citations. REVIEW CITATIONS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 14 citations