Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine

@article{Nawaz1998IsolationAC,
  title={Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine},
  author={Mohamed S. Nawaz and Daosheng Zhang and Ashraf A. Khan and Carl Cerniglia},
  journal={Applied Microbiology and Biotechnology},
  year={1998},
  volume={50},
  pages={568-572}
}
A gram-negative, rod-shaped bacterium capable of utilizing l-asparagine as its sole source of carbon and nitrogen was isolated from soil and identified as Enterobacter cloacae. An intracellularly expressed l-asparaginase was detected and it deaminated l-asparagine to aspartic acid and ammonia. High-pressure liquid chromatography analysis of a cell-free asparaginase reaction mixture indicated that 2.8 mM l-asparagine was hydrolyzed to 2.2 and 2.8 mM aspartic acid and ammonia, respectively… CONTINUE READING
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