Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine

  title={Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine},
  author={Mohamed S. Nawaz and Daosheng Zhang and Ashraf A. Khan and Carl Cerniglia},
  journal={Applied Microbiology and Biotechnology},
A gram-negative, rod-shaped bacterium capable of utilizing l-asparagine as its sole source of carbon and nitrogen was isolated from soil and identified as Enterobacter cloacae. An intracellularly expressed l-asparaginase was detected and it deaminated l-asparagine to aspartic acid and ammonia. High-pressure liquid chromatography analysis of a cell-free asparaginase reaction mixture indicated that 2.8 mM l-asparagine was hydrolyzed to 2.2 and 2.8 mM aspartic acid and ammonia, respectively… CONTINUE READING
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Publications referenced by this paper.
Showing 1-10 of 27 references

A speci®c Lasparaginase from Thermus aquaticus

MP Curran, RM Daniel, GR Guy, HW Moran
Arch Biochem Biophys • 1985
View 5 Excerpts
Highly Influenced

L-Asparagainases from Citrobacter freundii.

Biochimica et biophysica acta • 1977
View 5 Excerpts
Highly Influenced

Antitumor enzyme: polyethylene glycol-modified asparaginase.

Annals of the New York Academy of Sciences • 1990
View 2 Excerpts
Highly Influenced

Characterization of L-asparaginase from Bacillus sp. isolated form an intertidal marine alga (Sargassum sp.)

BR Mohapatra, RK Sani, UC Banerjee
Lett Appl Microbiol • 1995
View 1 Excerpt

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