Isolation and characterization of chromosome‐specific DNA sequences from a chromosome arm genomic library of common wheat

@article{Liu1997IsolationAC,
  title={Isolation and characterization of chromosome‐specific DNA sequences from a chromosome arm genomic library of common wheat},
  author={B. Liu and G. Segal and J. Vega and M. Feldman and S. Abbo},
  journal={Plant Journal},
  year={1997},
  volume={11},
  pages={959-965}
}
Summary Isolation, physical mapping and polymorphism of chromosome-specific DNA sequences in wheat are reported. Following the microdissection of the long arm of chromosome 5B (5BL) of common wheat, its DNA was amplified by degenerate oligonucleotide-primed PCR and directly cloned into plasmid vectors. Characterization of the chromosome arm library showed that ~55% of the inserts are of low-copy nature. Southern analysis using aneuploid lines of common wheat revealed that five of 11 low-copy… Expand

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