• Biology, Medicine
  • Published in
    The Journal of biological…
    1993

Isolation and characterization of DNA polymerase epsilon from the silk glands of Bombyx mori.

@article{Niranjanakumari1993IsolationAC,
  title={Isolation and characterization of DNA polymerase epsilon from the silk glands of Bombyx mori.},
  author={Somashekarappa Niranjanakumari and Karumathil P. Gopinathan},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 21},
  pages={
          15557-64
        }
}
The silk gland of Bombyx mori, an endomitotically replicative tissue shows high levels of DNA polymerases alpha, delta, and epsilon activities. The ratio of polymerase alpha to that of delta plus epsilon is maintained at 1.1 to 1.3 in both the posterior and middle silk glands for the entire duration of late larval development. The three activities copurify in the initial stages of fractionation through phosphocellulose and DE52 but polymerase alpha gets resolved from the others on… CONTINUE READING