Isolation and characterization of Cu/Zn-superoxide dismutase in Fasciola gigantica.

  title={Isolation and characterization of Cu/Zn-superoxide dismutase in Fasciola gigantica.},
  author={Hniang Lalrinkima and Opinder Krishen Raina and Dinesh Chandra and Siju Susan Jacob and Ram Krishna Bauri and Deepak Kumar Semwal and Hitender Singh Yadav and Mithilesh Singh and Ajayta Rialch and Anju Elsa Varghese and Prof. Dr. Sumit Kumar Banerjee and Navneet Kaur and Ashish Sharma},
  journal={Experimental parasitology},
A full-length complementary DNA (cDNA) encoding Cu/Zn-superoxide dismutase was isolated from Fasciola gigantica that on nucleotide sequencing showed a close homology (98.9%) with Cu/Zn-superoxide dismutase (SOD) of the temperate liver fluke, F. hepatica. Expression of the gene was found in all the three developmental stages of the parasite viz. adult, newly excysted juvenile and metacercaria at transcriptional level by reverse transcription-polymerase chain reaction (RT-PCR) and at the protein… CONTINUE READING