Isolation and characterization of CYP6B4, a furanocoumarin-inducible cytochrome P450 from a polyphagous caterpillar (Lepidoptera:papilionidae).

@article{Hung1997IsolationAC,
  title={Isolation and characterization of CYP6B4, a furanocoumarin-inducible cytochrome P450 from a polyphagous caterpillar (Lepidoptera:papilionidae).},
  author={Chi F Hung and May R. Berenbaum and Mary A. Schuler},
  journal={Insect biochemistry and molecular biology},
  year={1997},
  volume={27 5},
  pages={
          377-85
        }
}
Papilio glaucus (tiger swallowtail) is a generalist that rarely encounters plants containing furanocoumarins yet is constitutively capable of metabolizing low levels of these highly toxic allelochemicals. In larvae of this species, metabolism of linear (xanthotoxin, bergapten), and angular (angelicin, sphondin), furanocoumarins can be induced up to 30-fold by the presence of xanthotoxin in their diet. Degenerate primers corresponding to conserved amino acid sequences in three insect P450s… Expand
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TLDR
CYP6B3 cDNA derived from a second locus that is evolutionarily related to the CYP6B1 locus is cloned and indicates that P. polyxenes has adapted to the presence of the wide range of furanocoumarins in its host plants by diversifying its P450 isozyme structures and its furanOCoumarin‐responsive regulatory cascades. Expand
Transcriptional regulation of the Papilio polyxenes CYP6B1 gene.
TLDR
RNase protection analyses on RNA from larvae induced by linear and angular furanocoumarins indicate that transcription of the CYP6B1 gene is induced in insects significantly in response to xanthotoxin and only slightly in responseto bergapten, concluding that this P450 gene is transcriptionally regulated in vivo by at least one of the substrates which the encoded protein metabolizes. Expand
Black swallowtail (Papilio polyxenes) alleles encode cytochrome P450s that selectively metabolize linear furanocoumarins.
TLDR
Biochemical purification of the P450 polypeptide induced in Papilio polyxenes larvae in response to xanthotoxin has allowed us to clone cDNAs encoding two allelic variants of the CYP6B1 locus, indicating that the active site of CYP 6B1 binds angular furanocoumarins. Expand
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Data indicate that a complex array of CYP6B1 transcripts, derived from a minimum of two loci, is responsible for furanocoumarin metabolism in P. polyxenes . Expand
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