Isolation and characterization of CD34+ hematopoietic stem cells from human peripheral blood by high-gradient magnetic cell sorting.

@article{Kato1993IsolationAC,
  title={Isolation and characterization of CD34+ hematopoietic stem cells from human peripheral blood by high-gradient magnetic cell sorting.},
  author={Kazuyoshi Kato and Andreas Radbruch},
  journal={Cytometry},
  year={1993},
  volume={14 4},
  pages={
          384-92
        }
}
CD34+ cells, present at a frequency of 0.18 +/- 0.052% among leukocytes from peripheral blood (PB), can be rapidly and efficiently (recovery of 39.0-74.0%) enriched to a frequency of 38.6-87.1% (54.4 +/- 12.3%) by high-gradient magnetic cell separation (MACS) for immunophenotyping, characterization in colony-forming cell (CFU) assays, and further purification to homogeneity (> 98%) by multiparameter fluorescence-activated cell sorting (FACS). Enriching PB-CD34+ cells for immunophenotyping… CONTINUE READING

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