Isolation and characterization of 16 microsatellite markers from a rare and endemic species, Scutellaria austrotaiwanensis (Lamiaceae)

@article{Hsu2009IsolationAC,
  title={Isolation and characterization of 16 microsatellite markers from a rare and endemic species, Scutellaria austrotaiwanensis (Lamiaceae)},
  author={Tsai-Wen Hsu and Chia-Chi Kuo and Chi-Chu Tsai and Yu‐Chung Chiang},
  journal={Conservation Genetics Resources},
  year={2009},
  volume={1},
  pages={85-88}
}
Scutellaria austrotaiwanensis is a new species first described in 1997. It is a rare and endemic species in southern Taiwan. In this study, 16 polymorphic microsatellite loci were isolated from Scutellaria austrotaiwanensis using a magnetic bead enrichment method. Several genetic indices, including observed heterozygosity (Ho), expected heterozygosity (He), number of effective alleles (Ne), and polymorphism information content (PIC), were calculated. Deviation of heterozygosity was detected by… 
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References

SHOWING 1-10 OF 23 REFERENCES
Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)
TLDR
Twenty polymorphic microsatellite loci were isolated from the Japanese scallop using a method of hybridizing twice and these newly isolated markers increase the available molecular resources of the JapaneseScallop.
Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)
TLDR
The polymorphic microsatellite DNA markers reported here should provide a helpful means to address questions concerning population structure and demographic history of P. polysyphonum for conservation efforts.
Genetic Characterization and Phytochemical Analysis of Wild and Cultivated Populations of Scutellaria baicalensis
TLDR
The results from genetic characterization and phytochemical analysis demonstrated that the quality variation of this drug was mainly determined by extrinsic environmental or agricultural factors, rather than by genetic differences.
Comparisons of Scutellaria baicalensis, Scutellaria lateriflora and Scutellaria racemosa: genome size, antioxidant potential and phytochemistry.
TLDR
Phytochemical analyses detected and quantified the flavonoids baicalin, baicalein, scutellarin, and wogonin as well as the human neurohormones melatonin and serotonin in leaf and stem tissues from S. racemosa, the first phytochemical analysis of S. Racemosa.
Arlequin (version 3.0): An integrated software package for population genetics data analysis
TLDR
A completely new graphical interface written in C++, a more robust semantic analysis of input files, and two new methods: a Bayesian estimation of gametic phase from multi-locus genotypes, and an estimation of the parameters of an instantaneous spatial expansion from DNA sequence polymorphism are introduced.
genalex 6: genetic analysis in Excel. Population genetic software for teaching and research
genalex is a user-friendly cross-platform package that runs within Microsoft Excel, enabling population genetic analyses of codominant, haploid and binary data. Allele frequency-based analyses
Tandem repeats finder: a program to analyze DNA sequences.
TLDR
A new algorithm for finding tandem repeats which works without the need to specify either the pattern or pattern size is presented and its ability to detect tandem repeats that have undergone extensive mutational change is demonstrated.
Rapid isolation of high molecular weight plant DNA.
TLDR
A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA.
Variability within and among natural populations
"Wright's views about population genetics and evolution are so fundamental and so comprehensive that every serious student must examine these books firsthand. . . . Publication of this treatise is a
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
TLDR
The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
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