Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana

@article{Doutriaux1998IsolationAC,
  title={Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana},
  author={M P Doutriaux and Florence Couteau and Catherine Bergounioux and Chris A. White},
  journal={Molecular and General Genetics MGG},
  year={1998},
  volume={257},
  pages={283-291}
}
By using RT-PCR and degenerate oligonucleotides based on the sequence homology between the yeast RAD51 and DMC1 genes, two genes belonging to the RAD51 and DMC1 families were isolated from Arabidopsis thaliana ecotype Columbia. A RAD51 genomic DNA was also sequenced which is almost identical to its Landsberg erecta counterpart, except for a few translationally silent substitutions and for the presence of a 527-bp element downstream of the polyadenylation site. This element is repeated in the… CONTINUE READING