Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger

@article{Ngiam1997IsolationAC,
  title={Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger},
  author={Celina Ngiam and David J. Jeenes and David B. Archer},
  journal={Current Genetics},
  year={1997},
  volume={31},
  pages={133-138}
}
Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccharomyces cerevisiae PDI gene as a probe. Sequencing of a genomic clone and RT-PCR products predict a 515-aa protein comprising a 20-aa ER-translocation signal sequence and a 495-aa… CONTINUE READING
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Isolation and characterisation of a stressinducible PDIfamily gene from Aspergillus ni

  • DJ Jeenes, R Pfaller, DB Archer
  • 1996

Isolation and characterisation of a yeast gene , MPD 1 , the over - expression of which suppresses inviability caused by protein disulphide isomerase depletion

  • H Tachikawa, Y Takeuchi, +5 authors K Onodera
  • FEBS Lett
  • 1995

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