Isolation and biological properties of osteopontin from bovine milk.

@article{Bayless1997IsolationAB,
  title={Isolation and biological properties of osteopontin from bovine milk.},
  author={Kayla J. Bayless and George E Davis and Gerald A. Meininger},
  journal={Protein expression and purification},
  year={1997},
  volume={9 3},
  pages={309-14}
}
A procedure for the isolation of osteopontin (OPN) from bovine milk using ion-exchange and hydrophobic chromatography is described. A DEAE-Sephacel column followed by dual phenyl-Sepharose columns yielded approximately 8 mg of purified protein per liter of milk. SDS-PAGE analysis revealed that the protein migrated at M(r) 60,000. NH2-terminal sequence analysis of the first seven amino acids revealed the protein to be identical to that previously reported for bovine OPN. Also, our preparation… CONTINUE READING