Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom.

@article{Bello2006IsolationAB,
  title={Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom.},
  author={C. A. Bello and A. L. Hermogenes and A. Magalh{\~a}es and S. S. Veiga and L. H. Gremski and M. Richardson and E. Sanchez},
  journal={Biochimie},
  year={2006},
  volume={88 2},
  pages={
          189-200
        }
}
In investigations aimed at characterizing snake venom clot-dissolving enzymes, we have purified a fibrinolytic proteinase from the venom of Bothrops leucurus (white-tailed jararaca). The proteinase was purified to homogeneity by a combination of molecular sieve chromatography on Sephacryl S-200 and ion-exchange chromatography on CM Sepharose. The enzyme called leucurolysin-a (leuc-a), is a 23 kDa metalloendopeptidase since it is inhibited by EDTA. PMSF, a specific serine proteinase inhibitor… Expand
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