Isolation and biochemical analysis of plant small RNAs.

  title={Isolation and biochemical analysis of plant small RNAs.},
  author={H. Alexander Ebhardt and Mariana Oviedo Ovando and Peter J. Unrau},
  journal={Methods in molecular biology},
Small RNAs, defined as noncoding 20-30-nt-long RNAs, are instrumental regulators of cellular processes in most eukaryotes. In this chapter we describe three methods for extracting small RNA from cells: a general method, one plant specific and a third particular to conifers. Further, protocols are given for the analysis and quantification of small RNAs using polyacrylamide gel-based approaches. A native streptavidin gel-shift assay, useful for measuring the relative amounts of multiple small… CONTINUE READING

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