Corpus ID: 30984422

Isolation and analysis of vitamin B 12 from plant samples

  title={Isolation and analysis of vitamin B 12 from plant samples},
  author={M. Nakos and Iliyana Pepelanova and Sascha Beutel and Ulrich Krings and Ralf G. Berger and Thomas Scheper},
Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient… Expand
3 Citations
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