Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane.

@article{Shimazaki2014IsolationAA,
  title={Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane.},
  author={Youji Shimazaki and Yu Hirose},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2014},
  volume={972},
  pages={53-7}
}
Monomeric molecules such as amyloid-β can aggregate and transform into oligomeric and fibrous forms, which are implicated in the development and progression of Alzheimer's disease. Novel analytical techniques for the formation of oligomers are required to examine the neurotoxic amyloid-β oligomers involving fibrils. After isolating amyloid-β monomer 1-42 using a biotinylated antibody bound to membrane-immobilized avidin (immunoaffinity membrane), their masses were determined by MALDI-TOF MS… CONTINUE READING