Isolation and air-liquid interface culture of human large airway and bronchiolar epithelial cells.

@article{Bals2004IsolationAA,
  title={Isolation and air-liquid interface culture of human large airway and bronchiolar epithelial cells.},
  author={Robert Bals and Christoph Beisswenger and Sabine Blouquit and Thierry Chinet},
  journal={Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society},
  year={2004},
  volume={3 Suppl 2},
  pages={49-51}
}
This article describes the techniques of isolation and culture of human airway epithelial cells from large airways and from distal airways. Both cell types are obtained from lung pieces collected during surgery. The protocols start with an initial step of washing and dissection of the lung pieces to separate large airways or distal airways from the surrounding parenchyma. The second step is enzymatic isolation of epithelial cells from the dissected large or distal airways. Cells are then… CONTINUE READING

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