Isolation and Sequence Analysis of Wheat Tissue-Specific cDNAs by Differential Display

Abstract

The differential display technology was used to study and isolate tissue-specific cDNA from wheat (Triticum aestivum). cDNA was synthesized by reverse transcription from total RNA from wheat leaves, anthers, and ovaries to search for and isolate tissue-specific cDNAs and use them to screen wheat genomic library to get the corresponding genomic DNA clone… (More)
DOI: 10.1007/s11105-010-0213-1

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