Corpus ID: 87745867

Isolation and Purification of Superoxide Dismutase from Radix Lithospermi Seed

@article{Qinsheng2003IsolationAP,
  title={Isolation and Purification of Superoxide Dismutase from Radix Lithospermi Seed},
  author={Yuan Qin-sheng},
  journal={Pharmaceutical biotechnology},
  year={2003}
}
Superoxide dismutase (SOD) (EC 1.15.1.1.) was purified to homogeneity, from Radix Lithospermi seeds (RLS). Lipid and other pigments were removed by super critical fluid extraction (SCF). Stepwise isolation and purification procedure consisted of ammonium sulphate precipitation, ion exchange chromatograpgy on diethyl ammo ethyl-52 and gel filtration using Sephadex G-100. Pure RLS Superoxide dismutase had a specific activity of about 1855 units per milligram protein and was purified 100-fold… Expand
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