Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: Carboxyl methyltransferase

  title={Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: Carboxyl methyltransferase},
  author={M. Ashby and P. Errada and V. L. Boyartchuk and J. Rine},
  • M. Ashby, P. Errada, +1 author J. Rine
  • Published 1993
  • Biology, Medicine
  • Yeast
  • We isolated a mutant defective in C‐terminal farnesyl cysteine:carboxyl methyltransferase activity from a screen for mutations causing a‐specific sterility. A genomic fragment was cloned from a yeast multi‐copy library that restored mating. Both the cloned gene and the sterile mutation were allelic to the STE14 gene. A ste14‐complementing 2·17 kb BamHI fragment subclone was sequenced and found to encode a 239 amino acid protein with a molecular weight of 27,887 Daltons. The hydrophobicity… CONTINUE READING
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