Isolation and Characterization of a Chitinase and its cDNA Clone from Rice

@article{Anuratha2012IsolationAC,
  title={Isolation and Characterization of a Chitinase and its cDNA Clone from Rice},
  author={C. S. Anuratha and Jenq-Kuen Huang and Arunabala Pingali and Subbaratnam Muthukrishnan},
  journal={Journal of Plant Biochemistry and Biotechnology},
  year={2012},
  volume={1},
  pages={5-10}
}
A 35 kD chitinase has been purified to apparent homogeneity from extracts of rice bran of cv New Bonnet by ammonium sulfate fractionation, chitin affinity chromatography, cation exchange chromatography on carboxymethyl cellulose and gel filtration. The purified enzyme has an isoelectric point of 8.8. The enzyme inhibited the growth of Rhizoctonia solani (the sheath blight pathogen), Trichoderma viride, T. harzianum, Fusarium graminaerum and F. culmorum in vitro. A cDNA clone for chitinase was… CONTINUE READING