Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)

@article{Zhan2009IsolationAC,
  title={Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)},
  author={Aibin Zhan and Yao Wang and Bonnie Brown and H. Wang},
  journal={International Journal of Molecular Sciences},
  year={2009},
  volume={10},
  pages={18 - 27}
}
To perform whole genome scanning for complex trait analysis, we isolated and characterized a total of 21 novel genomic-SSRs and EST-SSRs for yellow perch (Perca flavescens), using the methods of construction of SSR-enrichment libraries and EST database mining of a related species P. fluviatilis. Of 16 genomic-SSR primer pairs examined, eight successfully amplified scorable products. The number of alleles at these informative loci varied from 3 - 14 with an average of 8.5 alleles per locus. When… CONTINUE READING
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